M-MulV Reverse Transcriptase

M-MulV Reverse Transcriptase

Product Details:

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Product Description

 

Description

M-MuLV Reverse Transcriptase (RT) is an RNA- and DNA-dependent DNA polymerase. The enzyme possesses a ribonuclease H activity specific to RNA in RNA-DNA hybrids (1, 2). M-MuLV RT incorporates modified nucleotides.

Source

E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

Applications

First strand cDNA synthesis for RT-PCR and real-time RT-PCR, see protocol on back page.

• Synthesis of cDNA for cloning and expression.

• Generation of labeled cDNA probes for microarrays.

• DNA labeling (3).

• Analysis of RNA by primer extension (3).

Rev.9



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