Description
M-MuLV Reverse Transcriptase (RT) is an RNA- and DNA-dependent DNA polymerase. The enzyme possesses a ribonuclease H activity specific to RNA in RNA-DNA hybrids (1, 2). M-MuLV RT incorporates modified nucleotides.
Source
E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR, see protocol on back page.
• Synthesis of cDNA for cloning and expression.
• Generation of labeled cDNA probes for microarrays.
• DNA labeling (3).
• Analysis of RNA by primer extension (3).
Rev.9