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    NdeI enzyme

    NdeI enzyme
    NdeI enzyme
    Product Code : ER0582
    Brand Name : GRACE LIFETECH
    Product Description


    1X Buffer O (for 100% NdeI digestion)

    50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 100 mM NaCl,

    0.1 mg/mL BSA.

    Incubation temperature


    Unit Definition

    One unit is defined as the amount of NdeI required to

    digest 1 μg of lambda DNA in 1 hour at 37°C in 50 μL of

    recommended reaction buffer.


    Dilute with Dilution Buffer (#B19): 10 mM Tris-HCl

    (pH 7.4 at 25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT,

    0.2 mg/mL BSA and 50% glycerol.

    Double Digests

    Thermo Scientific Tango Buffer is provided to simplify buffer

    selection for double digests. 98% of Thermo Scientific

    restriction enzymes are active in a 1X or 2X concentration

    of Tango Buffer. Please refer to

    the best buffer for your experiments.

    1X Tango Buffer: 33 mM Tris-acetate (pH 7.9 at 37°C),

    10 mM magnesium acetate, 66 mM potassium acetate,

    0.1 mg/mL BSA.


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