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    Home » Products » Molecular Biology Reagents and Kits » M-MulV Reverse Transcriptase

    M-MulV Reverse Transcriptase

    M-MulV Reverse Transcriptase
    M-MulV Reverse Transcriptase
    Product Code : EP0351
    Brand Name : GRACE LIFETECH
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    Product Description

     

    Description

    M-MuLV Reverse Transcriptase (RT) is an RNA- and DNA-dependent DNA polymerase. The enzyme possesses a ribonuclease H activity specific to RNA in RNA-DNA hybrids (1, 2). M-MuLV RT incorporates modified nucleotides.

    Source

    E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

    Applications

    First strand cDNA synthesis for RT-PCR and real-time RT-PCR, see protocol on back page.

    • Synthesis of cDNA for cloning and expression.

    • Generation of labeled cDNA probes for microarrays.

    • DNA labeling (3).

    • Analysis of RNA by primer extension (3).

    Rev.9


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